pBR322 vectors having tetracycline-dependent replication
نویسندگان
چکیده
منابع مشابه
Tetracycline resistance element of pBR322 mediates potassium transport.
The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general for elements that increase resistance to tetracycline.
متن کاملSite-dependent cleavage of pBR322 DNA by restriction endonuclease HinfI.
Cleavage of pBR322 DNA I by the restriction endonuclease HinfI is preferentially inhibited at specific HinfI cleavage sites. These sites in pBR322 DNA I have been identified and ordered with respect to the frequency with which they are cleaved. The HinfI site most resistant to cleavage in pBR322 DNA I is unique in that runs of G-C base pairs are immediately adjacent on both sites. Two different...
متن کاملAttempt to Construct a Rop pUC19 from pBR322 Mutant lacking the Tetracycline Resistance Gene
The two commonly used plasmid vectors, pBR322 and pUC19, are both derivatives of the parent plasmid, pColE-1. However, it has been observed that pBR322 is excluded from the host cells following co-transformation of pBR322 and pUC19. One potential explanation for this exclusion effect is the presence of a functional rop gene in pBR322. The rop gene encodes for a protein that stabilizes the inter...
متن کاملTopoisomerase I mutants: the gene on pBR322 that encodes resistance to tetracycline affects plasmid DNA supercoiling.
Plasmid pBR322 DNA isolated from topoisomerase I mutants of Escherichia coli and Salmonella typhimurium exhibits a distinctive supercoiling distribution characterized by an extremely heterogeneous distribution of linking numbers that contains highly negatively supercoiled topoisomers. Analysis of the supercoiling distributions of deletion and insertion derivatives of pBR322 shows that the prese...
متن کاملThe Effects of Ampicillin versus Tetracycline on the Plasmid Copy Numbers of pBR322
The pBR322 plasmid contains genes encoding for tetracycline and ampicillin resistance. Escherichia coli DH5α cells with pBR322 plasmid were grown and plated on selective plates containing 1) ampicillin only and 2) tetracycline only with varying antibiotic concentrations of half strength, three-fourth strength and full strength (15 μg/mL tetracycline and 50 μg/mL ampicillin for full strength). C...
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ژورنال
عنوان ژورنال: Plasmid
سال: 2016
ISSN: 0147-619X
DOI: 10.1016/j.plasmid.2016.02.004